Koprov

Since when does acetone dissolve PLA? For PLA one should use ethyl acetate or chloroform

This is the same guy who says Poland doesn’t need to allow people in if they don’t want to, so yeeeah… Karma, bitch! :D

That really depends on your budget.

What you need is a plasmid (a peace of DNA) with all of the machinery needed for cobalamin production on it. You can do in-silico modeling to get the plasmid sequence but then you will need to make it. In order to make a plasmid you can either do all the wetwork yourself, which requires a lot of equipment and reagents or you can pay a company to synthesize do it for you.

Once you have a plasmid the rest if pretty straightforward: get the plasmid into bacteria (can be done with ice and calcium salts), get the bacteria into a container with sterile media (a fancy chicken broth with salts) and let them rip :)

Finally, you will need to isolate your cobalamin form the used media, but you’ll need to look into that, I haven’t done it before (filtration and fractioning most likely) ;)

If you need specific guidance on the process and materials to do it at home then look into biohacking and DIY Science communities, maybe there is even a physical one somewhere nearby if you are lucky! ;)

Good luck and take care!

Meh, f*ck’em :)

That’s insanity! It’s literally building a pile of TNT to sit upon and than handing everyone a match “for safety”!

Before you go, why not take one of them with you? Just a thought…

All part of a plan called Seven Mountains Mandate: https://en.wikipedia.org/wiki/Seven_Mountain_Mandate

They know they f’ed up badly and this is their only chance for a long while, so they are going all in.